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Image Search Results
Journal: bioRxiv
Article Title: Environmental influences on placental programming and offspring outcomes following maternal immune activation
doi: 10.1101/729228
Figure Lengend Snippet: A) Illustration of maternal blood collection, embryonic tissue collection and PCR to determine fetal sex. Lane (L) 1; molecular weight ladder. L2 is the ‘known’ male (M) control sample, confirmed by the presence of the top band ( Sry gene). L3 is the ‘known’ female (F) control, as depicted by an absence of Sry . L4 = female; L5-7 = males. Maternal plasma levels of B) corticosterone (pg/mL) and C) IL-1β (pg/mL) were elevated 3 h post LPS, validating maternal immune activation. Placental levels of D,G) Hsd11b1 , E,H) Hsd11b2 , F,I) Hsd11b2 / Hsd11b1 , J,N) OGT , K,O) DNMT1 , L,P) DNMT3a , M,Q) MECP2 , 3 h and 24 h post LPS. Group designations: SD-Saline (open squares), SD-LPS (closed squares), EE-Saline (open circles), EE-LPS (closed circles). Error bars represent mean ± SEM, n = 7 per sex/housing/MIA group. If there were no significant effects of sex, male and female offspring data were collapsed together for visualization. *p < 0.05, ** p < 0.01, *** p < 0.001, ****p <0.0001, LPS vs saline; a p < 0.05, aa p < 0.01, aaa p < 0.001, aaaa p <0.0001, male vs female; b p < 0.05, bb p < 0.01, bbb p < 0.001, bbbb p <0.0001, standard housing vs environmental enrichment; ; & p < 0.05, && p < 0.01, &&& p < 0.001, &&&& p <0.0001, SD-Saline vs SD-LPS; # p < 0.05, ## p < 0.01, SD-LPS vs EE-LPS. Fetal brain data are presented in Supplementary Table 2 .
Article Snippet: Gene expression changes in IL-1β (Rn00580432_m1), glucocorticoid receptor ( Nr3c1 ; Rn00561369_m1), FK506 binding protein 5 ( Fkbp5 ; Rn01768371_m1), mineralcorticoid receptor ( Nr3c2 ; Rn00565562_m1), 11-beta hydroxysteroid dehydrogenase type 1 ( Hsd11b1 ; Rn00567167_m1), 11-beta hydroxysteroid dehydrogenase type 2 ( Hsd11b2 ; Rn00492539_m1), corticotrophin releasing hormone ( Crh ; Rn01462137_m1), corticotrophin releasing hormone receptor type 1 ( Crhr1 ; Rn00578611_m1), corticotrophin releasing hormone receptor type 2 ( Crhr2 ; Rn00575617_m1), μ-opioid receptor ( Oprm1 ; Rn01430371_m1), DNA methyltransferase 1 ( DNMT1 ; Rn00709664_m1), DNA methyltransferase 3 alpha ( DNMT3a ;
Techniques: Molecular Weight, Control, Clinical Proteomics, Activation Assay, Saline